Review

st2l (R&D Systems)

R&D Systems is a verified supplier

R&D Systems manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

R&D Systems st2l

IL-33 and <t>ST2L</t> are overexpressed in human GC tissues and predict poor prognosis. a QRT-PCR analysis of IL-33 mRNA expression in GC and corresponding normal tissues ( n = 18). b Histogram displaying the relative mRNA expression of IL-33 in 18 GC tissues. Data are shown as −ΔΔCt and 2 −ΔCt . c IHC staining of α-SMA, IL-33 and ST2L in GC tissues (200×; scale bar = 100 μm). d Histogram displaying the number of α-SMA, IL-33 and ST2L positive cells/field in GC tissues. e Histogram displaying the correlation between IL-33 expression and ST2L expression determined by IHC ( r = 0.6503). f , g Survival of patients with low or high levels of IL-33 and ST2L protein expression. h – j Immunofluorescence staining of IL-33, α-SMA, FAP, ST2L and Cytokeratin in GC tissues (200×; scale bar = 400 μm). k – m Histogram displaying the percentage of IL-33 + /α-SMA + , IL-33 + /FAP + and ST2L + /Cytokeratin ++ cells in GC tissues. Data are represented as the mean ± SD; * P <0.05, ** P <0.01, *** P <0.001

St2l, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+human+st2l/Human+ST2%2FIL-33R+Antibody/pmc07018661-195-4-22
Average 93 stars, based on 31 article reviews

st2l - by Bioz Stars, 2026-09

93/100 stars

Images

1) Product Images from "The reciprocal interaction between tumor cells and activated fibroblasts mediated by TNF-α/IL-33/ST2L signaling promotes gastric cancer metastasis"

Article Title: The reciprocal interaction between tumor cells and activated fibroblasts mediated by TNF-α/IL-33/ST2L signaling promotes gastric cancer metastasis

Journal: Oncogene

doi: 10.1038/s41388-019-1078-x

IL-33 and ST2L are overexpressed in human GC tissues and predict poor prognosis. a QRT-PCR analysis of IL-33 mRNA expression in GC and corresponding normal tissues ( n = 18). b Histogram displaying the relative mRNA expression of IL-33 in 18 GC tissues. Data are shown as −ΔΔCt and 2 −ΔCt . c IHC staining of α-SMA, IL-33 and ST2L in GC tissues (200×; scale bar = 100 μm). d Histogram displaying the number of α-SMA, IL-33 and ST2L positive cells/field in GC tissues. e Histogram displaying the correlation between IL-33 expression and ST2L expression determined by IHC ( r = 0.6503). f , g Survival of patients with low or high levels of IL-33 and ST2L protein expression. h – j Immunofluorescence staining of IL-33, α-SMA, FAP, ST2L and Cytokeratin in GC tissues (200×; scale bar = 400 μm). k – m Histogram displaying the percentage of IL-33 + /α-SMA + , IL-33 + /FAP + and ST2L + /Cytokeratin ++ cells in GC tissues. Data are represented as the mean ± SD; * P <0.05, ** P <0.01, *** P <0.001

Figure Legend Snippet: IL-33 and ST2L are overexpressed in human GC tissues and predict poor prognosis. a QRT-PCR analysis of IL-33 mRNA expression in GC and corresponding normal tissues ( n = 18). b Histogram displaying the relative mRNA expression of IL-33 in 18 GC tissues. Data are shown as −ΔΔCt and 2 −ΔCt . c IHC staining of α-SMA, IL-33 and ST2L in GC tissues (200×; scale bar = 100 μm). d Histogram displaying the number of α-SMA, IL-33 and ST2L positive cells/field in GC tissues. e Histogram displaying the correlation between IL-33 expression and ST2L expression determined by IHC ( r = 0.6503). f , g Survival of patients with low or high levels of IL-33 and ST2L protein expression. h – j Immunofluorescence staining of IL-33, α-SMA, FAP, ST2L and Cytokeratin in GC tissues (200×; scale bar = 400 μm). k – m Histogram displaying the percentage of IL-33 + /α-SMA + , IL-33 + /FAP + and ST2L + /Cytokeratin ++ cells in GC tissues. Data are represented as the mean ± SD; * P <0.05, ** P <0.01, *** P <0.001

Techniques Used: Quantitative RT-PCR, Expressing, Immunohistochemistry, Immunohistochemistry-Resin, Immunofluorescence, Staining

Figure Legend Snippet: Relationship between IL-33 and ST2L expression levels and clinicopathologic parameters in 134 GC tissues

Techniques Used: Expressing, Immunostaining

CAFs-derived IL-33 promotes the migration, invasion and EMT of GC cells. a – d The migration and invasion ability of SGC7901 and MKN45 cells were analyzed after culture in medium alone (blank) or treatment with: exogenous IL-33 (300 ng/ml); co-culture with CAFs supplemented with IgG isotype antibody (3 μg/ml) or IL-33 neutralizing antibody (3 μg/ml). Histograms show the average cell number/field (100×; scale bar = 100 μm). e – h The migration and invasion ability of SGC7901 and MKN45 cells was detected after culture in medium alone (blank), co-culture with CAFs transfected with IL-33/siRNA or nc/siRNA. Histograms display the average cell number/field (100×; scale bar = 100 μm). i – l The migration and invasion ability of SGC7901 and MKN45 cells was analyzed after culture in medium alone (blank) or stimulation with exogenous IL-33 (300 ng/ml) plus IgG isotype antibody or ST2L neutralizing antibody (3 μg/ml); co-culture with CAFs in the presence of IgG isotype antibody (3 μg/ml) or ST2L neutralizing antibody (3 μg/ml). Histograms show the average cell number/field. (100×; scale bar = 100 μm). m , n QRT-PCR of the genes for EMT in SGC7901 and MKN45 cells after culture in medium alone (blank); or activation with exogenous IL-33 (300 ng/ml); co-culture with CAFs in the presence of IgG isotype antibody (3 μg/ml) or IL-33 neutralizing antibody (3 μg/ml). o – r Western blot of EMT markers in SGC7901 and MKN45 cells cultured in medium alone (blank); or stimulated with exogenous IL-33 (300 ng/ml) or co-culture with CAFs or co-cultured with CAFs in the presence of DMSO, U0126 (20 μM), IL-33 neutralizing antibody (3 μg/ml) or ST2L neutralizing antibody (3 μg/ml). p and r Densitometric analysis shows the expression level of EMT markers in GC cells stimulated by the above factors. Data are represented as the mean ± SD of three independent experiments; * P <0.05, ** P <0.01, *** P <0.001

Figure Legend Snippet: CAFs-derived IL-33 promotes the migration, invasion and EMT of GC cells. a – d The migration and invasion ability of SGC7901 and MKN45 cells were analyzed after culture in medium alone (blank) or treatment with: exogenous IL-33 (300 ng/ml); co-culture with CAFs supplemented with IgG isotype antibody (3 μg/ml) or IL-33 neutralizing antibody (3 μg/ml). Histograms show the average cell number/field (100×; scale bar = 100 μm). e – h The migration and invasion ability of SGC7901 and MKN45 cells was detected after culture in medium alone (blank), co-culture with CAFs transfected with IL-33/siRNA or nc/siRNA. Histograms display the average cell number/field (100×; scale bar = 100 μm). i – l The migration and invasion ability of SGC7901 and MKN45 cells was analyzed after culture in medium alone (blank) or stimulation with exogenous IL-33 (300 ng/ml) plus IgG isotype antibody or ST2L neutralizing antibody (3 μg/ml); co-culture with CAFs in the presence of IgG isotype antibody (3 μg/ml) or ST2L neutralizing antibody (3 μg/ml). Histograms show the average cell number/field. (100×; scale bar = 100 μm). m , n QRT-PCR of the genes for EMT in SGC7901 and MKN45 cells after culture in medium alone (blank); or activation with exogenous IL-33 (300 ng/ml); co-culture with CAFs in the presence of IgG isotype antibody (3 μg/ml) or IL-33 neutralizing antibody (3 μg/ml). o – r Western blot of EMT markers in SGC7901 and MKN45 cells cultured in medium alone (blank); or stimulated with exogenous IL-33 (300 ng/ml) or co-culture with CAFs or co-cultured with CAFs in the presence of DMSO, U0126 (20 μM), IL-33 neutralizing antibody (3 μg/ml) or ST2L neutralizing antibody (3 μg/ml). p and r Densitometric analysis shows the expression level of EMT markers in GC cells stimulated by the above factors. Data are represented as the mean ± SD of three independent experiments; * P <0.05, ** P <0.01, *** P <0.001

Techniques Used: Derivative Assay, Migration, Co-Culture Assay, Transfection, Quantitative RT-PCR, Activation Assay, Western Blot, Cell Culture, Expressing

The metastatic potential of GC cells induced by CAFs-derived IL-33 is mediated by the activation of ERK1/2-SP1-ZEB2 pathway via ST2L. a – d Western blot analysis showing the phosphorylation of ERK1/2 in SGC7901 and MKN45 cells treated with medium alone or stimulated as follows: exogenous IL-33 (300 ng/ml), U0126 (20 μM), CAFs supernatants (CAFsu) supplemented with IgG isotype control antibody (3 μg/ml), DMSO; co-cultured with CAFs transfected with IL-33/siRNA or NC/siRNA; CAFsu supplemented with IL-33 neutralizing antibody (3 μg/ml). e Western blot analysis of pERK1/2 in SGC7901 and MKN45 cells incubated with medium alone; exogenous IL-33 (300 ng/ml); U0126 (20 μM); co-culture with CAFs; IL-33 neutralizing antibody (3 μg/ml); and/or ST2L neutralizing antibody (3 μg/ml). f , g The migration and invasion ability of SGC7901 and MKN45 cells after culture in medium alone (blank) or stimulation with exogenous IL-33 (300 ng/ml) and/or U0126 (20 μM); co-culture with CAFs and/or U0126 (20 μM). Histograms show the average cell number per field. (100×; scale bar = 100 μm). h , i QRT-PCR analysis of SP1 mRNA expression in SGC7901 and MKN45 cells incubated in medium alone or stimulation with exogenous IL-33 (300 ng/ml) or CAFs in the presence of IgG isotype control antibody (3 μg/ml) or IL-33 neutralizing antibody (3 μg/ml). j , k Western blot analysis of pERK1/2, ERK1/2, SP1 and ZEB2 in SGC7901 and MKN45 cells incubated in medium alone or stimulated by co-culture with CAFs in the presence of exogenous IL-33 (300 ng/ml) or U0126 (20 μM). l Western blot analysis showing the protein expression of pERK1/2, ERK1/2, SP1 and ZEB2 in SGC7901 and MKN45 cells cultured in medium alone (Mock); or transfected with control plasmid (nc) or SP1-expressing plasmid. m Dual luciferase reporters containing four different lengths of the ZEB2 promoter were co-transfected with SP1-expressing plasmid into 293T cells. Firefly luciferase activity was assessed relative to Renilla luciferase activity. Data are representative of three independent experiments. Densitometry shows relative protein expression. * P <0.05, ** P <0.01, *** P <0.001

Figure Legend Snippet: The metastatic potential of GC cells induced by CAFs-derived IL-33 is mediated by the activation of ERK1/2-SP1-ZEB2 pathway via ST2L. a – d Western blot analysis showing the phosphorylation of ERK1/2 in SGC7901 and MKN45 cells treated with medium alone or stimulated as follows: exogenous IL-33 (300 ng/ml), U0126 (20 μM), CAFs supernatants (CAFsu) supplemented with IgG isotype control antibody (3 μg/ml), DMSO; co-cultured with CAFs transfected with IL-33/siRNA or NC/siRNA; CAFsu supplemented with IL-33 neutralizing antibody (3 μg/ml). e Western blot analysis of pERK1/2 in SGC7901 and MKN45 cells incubated with medium alone; exogenous IL-33 (300 ng/ml); U0126 (20 μM); co-culture with CAFs; IL-33 neutralizing antibody (3 μg/ml); and/or ST2L neutralizing antibody (3 μg/ml). f , g The migration and invasion ability of SGC7901 and MKN45 cells after culture in medium alone (blank) or stimulation with exogenous IL-33 (300 ng/ml) and/or U0126 (20 μM); co-culture with CAFs and/or U0126 (20 μM). Histograms show the average cell number per field. (100×; scale bar = 100 μm). h , i QRT-PCR analysis of SP1 mRNA expression in SGC7901 and MKN45 cells incubated in medium alone or stimulation with exogenous IL-33 (300 ng/ml) or CAFs in the presence of IgG isotype control antibody (3 μg/ml) or IL-33 neutralizing antibody (3 μg/ml). j , k Western blot analysis of pERK1/2, ERK1/2, SP1 and ZEB2 in SGC7901 and MKN45 cells incubated in medium alone or stimulated by co-culture with CAFs in the presence of exogenous IL-33 (300 ng/ml) or U0126 (20 μM). l Western blot analysis showing the protein expression of pERK1/2, ERK1/2, SP1 and ZEB2 in SGC7901 and MKN45 cells cultured in medium alone (Mock); or transfected with control plasmid (nc) or SP1-expressing plasmid. m Dual luciferase reporters containing four different lengths of the ZEB2 promoter were co-transfected with SP1-expressing plasmid into 293T cells. Firefly luciferase activity was assessed relative to Renilla luciferase activity. Data are representative of three independent experiments. Densitometry shows relative protein expression. * P <0.05, ** P <0.01, *** P <0.001

Techniques Used: Derivative Assay, Activation Assay, Western Blot, Phospho-proteomics, Control, Cell Culture, Transfection, Incubation, Co-Culture Assay, Migration, Quantitative RT-PCR, Expressing, Plasmid Preparation, Luciferase, Activity Assay

Knockdown of IL-33 expression in CAFs or ST2L expression in GC cells inhibits peritoneal dissemination of GC cells in nude mice. a , c In vivo tumor peritoneal dissemination was examined by co-injection of SGC7901 or MKN45 cells and CAFs transfected with control siRNA (NC) or IL-33 siRNA into nude mice. Metastatic nodules are indicated by the red arrows. b , d Histograms displaying the number of peritoneal nodules in nude mice. e – h In vivo tumor peritoneal dissemination was examined by co-injection of CAFs and GC cells with ST2L knockdown (KD) into nude mice. Metastatic nodules are indicated by the red arrows. f , h Histograms displaying the number of peritoneal nodules in nude mice. Data are representative of three independent experiments. * P <0.05, * P <0.05, ** P <0.01

Figure Legend Snippet: Knockdown of IL-33 expression in CAFs or ST2L expression in GC cells inhibits peritoneal dissemination of GC cells in nude mice. a , c In vivo tumor peritoneal dissemination was examined by co-injection of SGC7901 or MKN45 cells and CAFs transfected with control siRNA (NC) or IL-33 siRNA into nude mice. Metastatic nodules are indicated by the red arrows. b , d Histograms displaying the number of peritoneal nodules in nude mice. e – h In vivo tumor peritoneal dissemination was examined by co-injection of CAFs and GC cells with ST2L knockdown (KD) into nude mice. Metastatic nodules are indicated by the red arrows. f , h Histograms displaying the number of peritoneal nodules in nude mice. Data are representative of three independent experiments. * P <0.05, * P <0.05, ** P <0.01

Techniques Used: Knockdown, Expressing, In Vivo, Injection, Transfection, Control

Model for the epithelial–stromal interactions in the tumor microenvironment of GC. a IL-33 released from CAFs promotes the migration, invasion and EMT of GC cells via the ST2L-ERK1/2-SP1-ZEB2 pathway. b GC cell-derived TNF-α upregulates IL-33 expression in CAFs via the TNFR2-NF-κB-IRF-1 pathway

Figure Legend Snippet: Model for the epithelial–stromal interactions in the tumor microenvironment of GC. a IL-33 released from CAFs promotes the migration, invasion and EMT of GC cells via the ST2L-ERK1/2-SP1-ZEB2 pathway. b GC cell-derived TNF-α upregulates IL-33 expression in CAFs via the TNFR2-NF-κB-IRF-1 pathway

Techniques Used: Migration, Derivative Assay, Expressing

Immunohistochemical staining:

Article Title: IL-33 induces protective effects in adipose tissue inflammation during obesity in mice
Article Snippet: Forty adipocytes were randomly chosen in each section and the mean adipocyte size was determined using Scion Image software at ×20 magnification. .. Immunohistochemical staining was performed using molecule-specific and isotype-control antibodies as negative controls: anti-mouse F4/80 (Clone CI:A3-1, Serotec) for macrophages; anti-mouse ST2 (MD Biosciences); anti-mouse IL-33 (R&D Systems); anti-human IL-33 (Axxora, NESSY-1); anti-human ST2L (MAB10041, R&D Systems). .. Staining was visualized using biotinylated secondary antibodies and detection with the ABC/DAB system or Avidin FITC (Vector).

Article Title: Interleukin-33 Induces Protective Effects in Adipose Tissue Inflammation During Obesity in Mice
Article Snippet: Forty adipocytes were randomly chosen in each section and the mean adipocyte size was determined using Scion Image software at x20 magnification. .. Immunohistochemical staining was performed using molecule-specific and isotype-control antibodies as negative controls: anti-mouse F4/80 (Clone CI:A3-1, Serotec) for macrophages; anti-mouse ST2 (MD Biosciences); antimouse IL-33 (R&D Systems); anti-human IL-33 (Axxora, NESSY-1); anti-human ST2L (MAB10041, R&D Systems). .. Staining was visualized using biotinylated secondary antibodies and detection with the ABC/DAB system or Avidin FITC (Vector).

Staining:

Image Search Results

Journal: Oncogene

Article Title: The reciprocal interaction between tumor cells and activated fibroblasts mediated by TNF-α/IL-33/ST2L signaling promotes gastric cancer metastasis

doi: 10.1038/s41388-019-1078-x

Figure Lengend Snippet: IL-33 and ST2L are overexpressed in human GC tissues and predict poor prognosis. a QRT-PCR analysis of IL-33 mRNA expression in GC and corresponding normal tissues ( n = 18). b Histogram displaying the relative mRNA expression of IL-33 in 18 GC tissues. Data are shown as −ΔΔCt and 2 −ΔCt . c IHC staining of α-SMA, IL-33 and ST2L in GC tissues (200×; scale bar = 100 μm). d Histogram displaying the number of α-SMA, IL-33 and ST2L positive cells/field in GC tissues. e Histogram displaying the correlation between IL-33 expression and ST2L expression determined by IHC ( r = 0.6503). f , g Survival of patients with low or high levels of IL-33 and ST2L protein expression. h – j Immunofluorescence staining of IL-33, α-SMA, FAP, ST2L and Cytokeratin in GC tissues (200×; scale bar = 400 μm). k – m Histogram displaying the percentage of IL-33 + /α-SMA + , IL-33 + /FAP + and ST2L + /Cytokeratin ++ cells in GC tissues. Data are represented as the mean ± SD; * P <0.05, ** P <0.01, *** P <0.001

Article Snippet: Anti-human IL-33 (no. AF3625), ST2L (no. AF523), TNF-α (no. AF-410-NA), TNFR1 (no. MAB225-100) and TNFR2 (no. MAB726-100) neutralizing antibodies were purchased from R&D Systems.

Techniques: Quantitative RT-PCR, Expressing, Immunohistochemistry, Immunohistochemistry-Resin, Immunofluorescence, Staining

Journal: Oncogene

Article Title: The reciprocal interaction between tumor cells and activated fibroblasts mediated by TNF-α/IL-33/ST2L signaling promotes gastric cancer metastasis

doi: 10.1038/s41388-019-1078-x

Figure Lengend Snippet: Relationship between IL-33 and ST2L expression levels and clinicopathologic parameters in 134 GC tissues

Techniques: Expressing, Immunostaining

Journal: Oncogene

Article Title: The reciprocal interaction between tumor cells and activated fibroblasts mediated by TNF-α/IL-33/ST2L signaling promotes gastric cancer metastasis

doi: 10.1038/s41388-019-1078-x

Figure Lengend Snippet: CAFs-derived IL-33 promotes the migration, invasion and EMT of GC cells. a – d The migration and invasion ability of SGC7901 and MKN45 cells were analyzed after culture in medium alone (blank) or treatment with: exogenous IL-33 (300 ng/ml); co-culture with CAFs supplemented with IgG isotype antibody (3 μg/ml) or IL-33 neutralizing antibody (3 μg/ml). Histograms show the average cell number/field (100×; scale bar = 100 μm). e – h The migration and invasion ability of SGC7901 and MKN45 cells was detected after culture in medium alone (blank), co-culture with CAFs transfected with IL-33/siRNA or nc/siRNA. Histograms display the average cell number/field (100×; scale bar = 100 μm). i – l The migration and invasion ability of SGC7901 and MKN45 cells was analyzed after culture in medium alone (blank) or stimulation with exogenous IL-33 (300 ng/ml) plus IgG isotype antibody or ST2L neutralizing antibody (3 μg/ml); co-culture with CAFs in the presence of IgG isotype antibody (3 μg/ml) or ST2L neutralizing antibody (3 μg/ml). Histograms show the average cell number/field. (100×; scale bar = 100 μm). m , n QRT-PCR of the genes for EMT in SGC7901 and MKN45 cells after culture in medium alone (blank); or activation with exogenous IL-33 (300 ng/ml); co-culture with CAFs in the presence of IgG isotype antibody (3 μg/ml) or IL-33 neutralizing antibody (3 μg/ml). o – r Western blot of EMT markers in SGC7901 and MKN45 cells cultured in medium alone (blank); or stimulated with exogenous IL-33 (300 ng/ml) or co-culture with CAFs or co-cultured with CAFs in the presence of DMSO, U0126 (20 μM), IL-33 neutralizing antibody (3 μg/ml) or ST2L neutralizing antibody (3 μg/ml). p and r Densitometric analysis shows the expression level of EMT markers in GC cells stimulated by the above factors. Data are represented as the mean ± SD of three independent experiments; * P <0.05, ** P <0.01, *** P <0.001

Techniques: Derivative Assay, Migration, Co-Culture Assay, Transfection, Quantitative RT-PCR, Activation Assay, Western Blot, Cell Culture, Expressing

Journal: Oncogene

Article Title: The reciprocal interaction between tumor cells and activated fibroblasts mediated by TNF-α/IL-33/ST2L signaling promotes gastric cancer metastasis

doi: 10.1038/s41388-019-1078-x

Figure Lengend Snippet: The metastatic potential of GC cells induced by CAFs-derived IL-33 is mediated by the activation of ERK1/2-SP1-ZEB2 pathway via ST2L. a – d Western blot analysis showing the phosphorylation of ERK1/2 in SGC7901 and MKN45 cells treated with medium alone or stimulated as follows: exogenous IL-33 (300 ng/ml), U0126 (20 μM), CAFs supernatants (CAFsu) supplemented with IgG isotype control antibody (3 μg/ml), DMSO; co-cultured with CAFs transfected with IL-33/siRNA or NC/siRNA; CAFsu supplemented with IL-33 neutralizing antibody (3 μg/ml). e Western blot analysis of pERK1/2 in SGC7901 and MKN45 cells incubated with medium alone; exogenous IL-33 (300 ng/ml); U0126 (20 μM); co-culture with CAFs; IL-33 neutralizing antibody (3 μg/ml); and/or ST2L neutralizing antibody (3 μg/ml). f , g The migration and invasion ability of SGC7901 and MKN45 cells after culture in medium alone (blank) or stimulation with exogenous IL-33 (300 ng/ml) and/or U0126 (20 μM); co-culture with CAFs and/or U0126 (20 μM). Histograms show the average cell number per field. (100×; scale bar = 100 μm). h , i QRT-PCR analysis of SP1 mRNA expression in SGC7901 and MKN45 cells incubated in medium alone or stimulation with exogenous IL-33 (300 ng/ml) or CAFs in the presence of IgG isotype control antibody (3 μg/ml) or IL-33 neutralizing antibody (3 μg/ml). j , k Western blot analysis of pERK1/2, ERK1/2, SP1 and ZEB2 in SGC7901 and MKN45 cells incubated in medium alone or stimulated by co-culture with CAFs in the presence of exogenous IL-33 (300 ng/ml) or U0126 (20 μM). l Western blot analysis showing the protein expression of pERK1/2, ERK1/2, SP1 and ZEB2 in SGC7901 and MKN45 cells cultured in medium alone (Mock); or transfected with control plasmid (nc) or SP1-expressing plasmid. m Dual luciferase reporters containing four different lengths of the ZEB2 promoter were co-transfected with SP1-expressing plasmid into 293T cells. Firefly luciferase activity was assessed relative to Renilla luciferase activity. Data are representative of three independent experiments. Densitometry shows relative protein expression. * P <0.05, ** P <0.01, *** P <0.001

Techniques: Derivative Assay, Activation Assay, Western Blot, Phospho-proteomics, Control, Cell Culture, Transfection, Incubation, Co-Culture Assay, Migration, Quantitative RT-PCR, Expressing, Plasmid Preparation, Luciferase, Activity Assay

Journal: Oncogene

Article Title: The reciprocal interaction between tumor cells and activated fibroblasts mediated by TNF-α/IL-33/ST2L signaling promotes gastric cancer metastasis

doi: 10.1038/s41388-019-1078-x

Figure Lengend Snippet: Knockdown of IL-33 expression in CAFs or ST2L expression in GC cells inhibits peritoneal dissemination of GC cells in nude mice. a , c In vivo tumor peritoneal dissemination was examined by co-injection of SGC7901 or MKN45 cells and CAFs transfected with control siRNA (NC) or IL-33 siRNA into nude mice. Metastatic nodules are indicated by the red arrows. b , d Histograms displaying the number of peritoneal nodules in nude mice. e – h In vivo tumor peritoneal dissemination was examined by co-injection of CAFs and GC cells with ST2L knockdown (KD) into nude mice. Metastatic nodules are indicated by the red arrows. f , h Histograms displaying the number of peritoneal nodules in nude mice. Data are representative of three independent experiments. * P <0.05, * P <0.05, ** P <0.01

Techniques: Knockdown, Expressing, In Vivo, Injection, Transfection, Control

Journal: Oncogene

Article Title: The reciprocal interaction between tumor cells and activated fibroblasts mediated by TNF-α/IL-33/ST2L signaling promotes gastric cancer metastasis

doi: 10.1038/s41388-019-1078-x

Figure Lengend Snippet: Model for the epithelial–stromal interactions in the tumor microenvironment of GC. a IL-33 released from CAFs promotes the migration, invasion and EMT of GC cells via the ST2L-ERK1/2-SP1-ZEB2 pathway. b GC cell-derived TNF-α upregulates IL-33 expression in CAFs via the TNFR2-NF-κB-IRF-1 pathway

Techniques: Migration, Derivative Assay, Expressing

ST2L expression is lower in colorectal cancer tissue relative to adjacent non-tumour tissue. ( A ) RNA was extracted and changes in IL-33, total ST2 and ST2L detected using qRT–PCR. ( B ) Immunohistochemical staining for IL-33, ST2 and ST2L was performed on paraffin-embedded tissue sections. Original magnification: × 20. Images shown are representative of the findings obtained. ( C ) Serum was obtained from healthy individuals and patients with CRC, and IL-33 and sST2 levels determined using ELISA.

Journal: British Journal of Cancer

Article Title: An antitumorigenic role for the IL-33 receptor, ST2L, in colon cancer

doi: 10.1038/bjc.2015.433

Figure Lengend Snippet: ST2L expression is lower in colorectal cancer tissue relative to adjacent non-tumour tissue. ( A ) RNA was extracted and changes in IL-33, total ST2 and ST2L detected using qRT–PCR. ( B ) Immunohistochemical staining for IL-33, ST2 and ST2L was performed on paraffin-embedded tissue sections. Original magnification: × 20. Images shown are representative of the findings obtained. ( C ) Serum was obtained from healthy individuals and patients with CRC, and IL-33 and sST2 levels determined using ELISA.

Article Snippet: The primary antibodies used were as follows: polyclonal anti-human anti-ST2L antibody (cat # NBP1-85251) Novus, (Littleton, CO, USA), anti-mouse anti-ST2 antibody (cat#ab25877) Abcam (Cambridge, UK), anti-human anti-IL-33 antibody (Nessy-1) (cat#ALX-804-840) Enzo Life Sciences (Farmingdale, NY, USA).

Techniques: Expressing, Quantitative RT-PCR, Immunohistochemical staining, Staining, Enzyme-linked Immunosorbent Assay

Journal: British Journal of Cancer

Article Title: An antitumorigenic role for the IL-33 receptor, ST2L, in colon cancer

doi: 10.1038/bjc.2015.433

Figure Lengend Snippet: ST2L expression in colon tumour cells

Techniques: Expressing

ST2L expression is progressively lower with increasing tumour stage. ( A ) Immunohistochemical staining for ST2L was performed on paraffin-embedded colonic tissue sections. Original magnification: × 20. Images shown are representative of the findings obtained for each stage. ( B ) The relationship between ST2L expression in CRC cells and clinical prognosis in patients with CRC was analysed by Kaplan–Meier analysis. Differences in survival curves were determined by the log-rank test.

Journal: British Journal of Cancer

Article Title: An antitumorigenic role for the IL-33 receptor, ST2L, in colon cancer

doi: 10.1038/bjc.2015.433

Figure Lengend Snippet: ST2L expression is progressively lower with increasing tumour stage. ( A ) Immunohistochemical staining for ST2L was performed on paraffin-embedded colonic tissue sections. Original magnification: × 20. Images shown are representative of the findings obtained for each stage. ( B ) The relationship between ST2L expression in CRC cells and clinical prognosis in patients with CRC was analysed by Kaplan–Meier analysis. Differences in survival curves were determined by the log-rank test.

Techniques: Expressing, Immunohistochemical staining, Staining

Pro-inflammatory cytokines induce the expression of ST2L but not IL-33 in colon cancer cells, with IL-33 inducing migration but not proliferation or invasion in vitro . ( A ) Cells were stimulated for 24 h with LPS, TNF α and PGE 2 as indicated, and changes in ST2L and IL-33 detected. Immunoblotting for β -actin was used as the loading control. Data shown are representative of three independent experiments. Cells were stimulated with IL-33 as indicated and changes in ( B ) proliferation assessed by BrdU incorporation, with EGF used as a positive control, ( C ) migration assessed using a modified Boyden chamber assay and ( D ) invasion assessed using a Matrigel invasion assay system. Data shown are mean±s.e.m. ( n =3).

Journal: British Journal of Cancer

Article Title: An antitumorigenic role for the IL-33 receptor, ST2L, in colon cancer

doi: 10.1038/bjc.2015.433

Figure Lengend Snippet: Pro-inflammatory cytokines induce the expression of ST2L but not IL-33 in colon cancer cells, with IL-33 inducing migration but not proliferation or invasion in vitro . ( A ) Cells were stimulated for 24 h with LPS, TNF α and PGE 2 as indicated, and changes in ST2L and IL-33 detected. Immunoblotting for β -actin was used as the loading control. Data shown are representative of three independent experiments. Cells were stimulated with IL-33 as indicated and changes in ( B ) proliferation assessed by BrdU incorporation, with EGF used as a positive control, ( C ) migration assessed using a modified Boyden chamber assay and ( D ) invasion assessed using a Matrigel invasion assay system. Data shown are mean±s.e.m. ( n =3).

Techniques: Expressing, Migration, In Vitro, Western Blot, Control, BrdU Incorporation Assay, Positive Control, Modification, Boyden Chamber Assay, Invasion Assay

Journal: The Journal of allergy and clinical immunology

Article Title: Phenotypic and genotypic association of epithelial IL1RL1 to human T H 2-like asthma

doi: 10.1016/j.jaci.2014.06.023

Figure Lengend Snippet: TABLE II

Article Snippet: The primary antibody used was a rabbit polyclonal anti-human ST2L antibody (Millipore, Billerica, Mass) at a 1:100 dilution.

Techniques:

Review

st2l (R&D Systems)

Structured Review

Images

1) Product Images from "The reciprocal interaction between tumor cells and activated fibroblasts mediated by TNF-α/IL-33/ST2L signaling promotes gastric cancer metastasis"

Related Articles

Immunohistochemical staining:

Staining:

Similar Products

Image Search Results